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18 Cards in this Set

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Water Dissociation Constant
Keq =
Keq= [H][OH]/[H2O]
pH=
pH=-log[H]
Bronstead Acid
Releases a proton
Bronstead Base
Accepts a proton
pKa=
pKa=-log(Ka)
Henderson-Hasselbalch Equation
pH =
pH = pKa + log([A-]/[Ha])
What is a polyprotic acid?
An acid that has more than one acid group
1st Law of Thermodynamics
Energy can be neither created nor destroyed
2nd Law of Thermodynamics
Universe is moving towards a constant increase in entropy (disorder)
Hess's Law
For a particular set of reactants going to a particular set of products, the Enthalpy change is the same regardless of steps
delta G^0 =
delta G^0 = -RT ln(Keq)
Keq =
Keq = e^(-delta G/RT)
Chromatography
Stationary phase and a mobile phase
Ion Exchange Chromatography (for anions)
Protein in anionic (-)
Resin is cationinc (+)
Protein binds to charged resin
Resin is washed to remove contaminates.
Protein is washed off with a stronger salt solution
Size Exclusion Chromatography
Larger molecules pass through faster
Affinity Chromatography
Specific binding of a component of the mixture. Other molecules pass through. Bound molecules are washed off later.
HPLC
Very fine separation of components
Electrophoresis
Uses the charge of the sample mixture to separate components
Positively charge molecules travel towards the cathode (-)